Journal: Biomaterials

Article Title: Self-assembled protein vesicles as vaccine delivery platform to enhance antigen-specific immune responses.

doi: 10.1016/j.biomaterials.2024.122666

Figure Lengend Snippet: Fig. 5. ELISA endpoint titers of OVA specific IgG1 (a) and IgG2a (b) antibodies after prime and boost immunization. Each mouse was immunized with 10 μg OVA-ZE in both soluble OVA-ZE group and OVA protein vesicle group. Titer values too low for detection were arbitrarily set at 10. (**p < 0.01).

Article Snippet: After serum incubation, plates were washed with PBST and incubated with HRP-conjugated goat antimouse IgG1 antibodies (Southern Biotech) or HRP-conjugated goat antimouse IgG2a-HRP antibodies (Southern Biotech) at a 1:4000 dilution in 0.1 % BSA/PBST for 1 h at room temperature followed by washing with PBST.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Biomaterials

Article Title: Self-assembled protein vesicles as vaccine delivery platform to enhance antigen-specific immune responses.

doi: 10.1016/j.biomaterials.2024.122666

Figure Lengend Snippet: Fig. 6. Comparison of ELISA endpoint titers of IgG1 (a) and IgG2a (b) against OVA antigen and pZR-ELP antigen. Titer values too low for detection were arbitrarily set at 10. Data labeled “Against OVA” in red is replotted from Fig. 5 to compare with the titers against pZR-ELP. (**p < 0.01, *p < 0.05). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: After serum incubation, plates were washed with PBST and incubated with HRP-conjugated goat antimouse IgG1 antibodies (Southern Biotech) or HRP-conjugated goat antimouse IgG2a-HRP antibodies (Southern Biotech) at a 1:4000 dilution in 0.1 % BSA/PBST for 1 h at room temperature followed by washing with PBST.

Techniques: Comparison, Enzyme-linked Immunosorbent Assay, Labeling